Yes. Most Open Pollinated seeds can cross each other.
Unless you are trying to breed a new variety, it is best to space them far apart.
I know a guy who raises peppers for seed, and doesn't have enough acreage to space 30+ varieties at the proper distance.
So, he built little cages, approx 4' x 4' x 4' with PVC pipe, and has them covered with shade cloth, to keep the pollinators confined to one variety only. The cages need to be opened before flowering to allow pollinators in, but then closed so they cannot move on to another bush.
If you decide to hand pollinate individual flowers, two methods are suggested: use an artist's brush to simulate a bee browsing around in the flower, or use a vibrator to cause the pollen to 'fly' (electric tooth brushes work well for this). Then, the selected flowers need to be identified for later collection. A small length of red
thread tied around the stem will do the trick.
For pepper seeds to be mature, the pepper needs to be
ripe. This means letting it remain on the vine until it turns red...usually beyond the eating stage.
Here is a chart which shows how the different pepper varieties will/might/won't cross pollinate: