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Homemade Lactobacillus Serum
This is a wonderful recipe, I use it daily in my own gut, in fermented foods, on the garden, in the animals water, it really has a million uses.

I did not write the following article, all credit goes to the author, but I am posting it here so people have a reference, the link will be at the bottom of the page.

Lactobacillus Serum

This is the workhorse of the beneficial bacteria we’ll be discussing here. We use it for everything! Foul odors, clogged drains, cheaper pig/chicken/etc farming, aquaculture, the applications are amazingly diverse. Learn how to make and use this and you will have a powerful tool in your farming arsenal.

How to Make:

Get container, fill halfway with rice-wash. Rice wash is the water leftover when you rinse fresh rice. For example, go buy rice, whatever kind, bring it home, put it in a pot with warm water, swirl it a bit and then drain the [now milky colored] water. The water is now a rich source of carbohydrates. In this step, you can substitute rice with another carbohydrate source if you don’t have rice, as long as it is complex (don’t use simple carbohydrates like sugar, honey, syrup, molasses, etc). You can use wheat, barley, kinoa, other carbohydrates as the base to make your carbohydrate wash. This wash will attract microbes from the air, among them lacto bacilli.
Cover loosely and let stand for a couple days to a week
When is it done? When you see a light film on top (molds) and it smells a little sour and forms 3 layers. This is indicating the rice wash is infected with various microbes. This happens more quickly in warm temperatures because microbes are more active. Thus it is all relative since we don’t do this in controlled laboratory conditions.
The layers are distinct
Top layer: floating carbohydrates leftover from fermentation and possibly molds
Middle layer: Lactic Acid and other bacteria (cheese buffs will recognize this as a makeshift “rennet”). We will use this layer.
Bottom layer: Starch, byproduct of fermentation
Extract the middle layer using a siphon. This layer contains the highest concentration of lactic acid bacteria and lowest concentration of the unneeded byproducts
Get a new container, larger than the first. Take the extracted serum from the last step and mix it with 10 parts milk. By saturating with milk (lactose), we dissuade other microbes from proliferating, leaving L. bacilli. E.G. if you have 1cup of the serum, mix it with 10cups milk.

TIP: The best milk to use in unpasteurized natural milk. However, any milk will do, even powdered milk. In our experience, the best is unpasteurized natural but just use what is available. We just want to saturate with lactose to promote L. bacilli bacteria.

You want to keep this stage anaerobic as much as possible. You can use something like rice bran, barley bran, wheat bran, etc sprinkled on top of the milk. I use a sealed container with a one-way valve. Note: Beware of bubbling during this phase. It can lead to overflows if you filled to near the top. It can go through the one-way valves so keep an eye on it and don’t do this step around nice things.
After about 1 week (temp dependent), you’ll see curds (made of carbohydrate, protein, and fat) on top of the milk. The water below will be yellow colored – this is whey, enriched with lactic acid bacteria from the fermentation of the milk.

NOTE: Microbes like L. bacilli are more active in warmer temperatures. The curds you see are a byproduct of the fermentation process. Fermentation is generally associated with microbial processes under anaerobic(no oxygen) conditions. Now, L. bacilli is a facultative anaerobe, that is it can live and work with or without oxygen, but less competition in anaerobic conditions.

The water below(whey+lacto) is the good stuff. You want to extract this. You can either skim the curds off the top, pour through a strainer, or whatever other methods to accomplish that

NOTE: Remember the curds, or byproduct of milk fermentation by L. bacilli, are great food. They are full of beneficial microbes like L. bacilli. Feed the curds to the soil, compost pile, plants, animals, humans – whoever wants them! They are full of good nutrients/microbes. No waste in natural farming.

To preserve at room temperature, add an equal part sugar/molasses to the serum. So, if you have 1L of serum, add 1kilo sugar or 1L molasses. Otherwise store in fridge to keep.

Example Recipe:

1 L rice wash
add 10L Milk
After rice wash and milk remove curds – around 1L
Left with 10L pure LAB (lactic acid bacteria)
add 10kg sugar or 10L molasses
= 20 L stabilized lactic acid bacteria serum

What to Use it for and How

Before using, first mix 1:20 with water. 1 part serum to 20 parts water. Then follow instructions below:

Odor Reducer:
Add mixture to animal’s water at 2tbsp/L. You can mix it more or less, there are no rules here, just how we typically do it.

Apply to places where there is odor buildup. The harmless bacteria “eat” the odor causing germs and the smell is gone!
Indoors: reduces foul odors, including animals like cats, dogs, mice, other pets. Stinky shoes? Wet clothes from being outside? Gym clothes that haven’t made it to the wash yet? Smoker in the house? Kill these nasty smells!
Outside: use to control odor in pens – pigs, cows, chickens. In barns, around the yard, etc

Household use:

Clear clogged drains: dump mixture into drain to clear clogs. Exact amount depends on the clog, haha. A few tbsp to 1L works well. For semi-clogged drains (like kitchen sink draining progressively slower), use at night and allow at least the night for microbes to work.
Keep septic clear. Tired of having your septic system drained? Add lacto! Depending on size of your system, pour a few tbsp. to a few L into the toilet every few months.
Houseplants: Mix 2-3tbsp per 1L water and use that to water them.

Animal Bedding:
Mix 2tbsp to 1L water. Mix with animal bedding to reduce smell and increase longevity. In natural pig farming we use at least 1 yard deep of bedding so there is plenty of space for microbes to work. Bedding consists of organic substrate like rice hulls, wood chips, sawdust, wood shavings, shredded corn cob, any other high cellulose, high lignin material. Natural pig farming is a future topic on this site. Spray until bedding is slightly damp but not wet. How much you spray really depends on your climate. If you are in a very dry climate you can spray a little more and mix in evenly. Wetter (more humid) climates use a bit less. Mix into the bedding evenly where necessary (in many cases, like with pigs and chickens, they’ll mix it themselves). How much you use is all relative. These guidelines are for pigs and chickens. More extreme smells, just use more! Want to spray less often, use more! As we notice a smell we spray. Thus, as pigs grow bigger, make more poop, we spray more often! Dosage/frequency is relative and will depend on your situation.

Animals – Digestive/Growth Aid:
Mix 2tbsp to 1L water, then add that mixture to animal’s water at 2tbsp/L(so the animal’s water contains little less than a quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so its just about adding enough to be effective, without wasting it.

Improve digestive efficiency in humans and animals alike:
Improves how you feel after meals, particularly meals rich in meats. It’s awesome. After eating, mix 1-2tbsp lacto with a cup of water and drink that. Makes you feel so much better after! Lessens that afternoon lull, gives you more energy!
Aids digestion in animals. This is critical. You can raise animals on less food, and see the same and greater growth rates. Amazing results in pigs . The principal is that the microorganisms help digest the food coming in – better digestibility means better nutrient absorption. Save on feeds, better feed to growth conversion ratio!

TIP: If you really want to boost growth, mix 2tbsp to 1L water and soak the food in this solution for a few hours to a few days. Food is pre-digested when animals eat it, AWESOME!

Great results in livestock and poultry.

Plants – Growth Aid:
When added to water for plants, nutrient uptake efficiency is increased, which increases growth!

Improves growth of plants when applied as foliar spray and soil drench. Improves their efficiency in uptaking nutrients so naturally, growth is enhanced. With the use of these microorganisms, the nutrients you spray or drench to feed your plants become more bio-available and easily absorbable by the plants. Technically, you can say that plants do not use organic nutrients directly. Microorganisms convert organic nutrients to their inorganic constituents which the plants utilize. Utilizing microbes, you will notice better plant growth and health.

Disease Resistance:

This is a consequence of the increased efficiency of nutrients. More nutrients available at smaller metabolic cost.
Lacto suppresses harmful bacteria in food/water that animals consume, enhances their gut flora so that line of defense is working optimally, etc.

Aid Compost:

Mix 2tbsp/L and spray on compost pile to improve decomposition. This is a huge topic that will be expanded upon in another post.

Aid Organic Fertilizer:
Add 1-2tbsp per gallon water-nutrient solution. Lacto consumes organic nutrients making them bio-available to plant roots.

Plants don’t use organic fertilizer! Microbes break it down to inorganic constituents, and plants take those up. This product makes that process more efficient.

Lacto works in aquaculture just fine if you don’t have BIM available. Add lacto at roughly 1L per 700m3 of fish-containing water. Example: you have a pond that averages 20m wide by 30m long by 2m deep. So, 20 x 30 x 2 = 1200m3. In this case you would add roughly 1L of BIM or Lacto

Microbes digest fish wastes, cleaning up water and improving water quality.
Allows fish to grow larger due to digestive efficiency
Allows higher population of fish in the same amount of water! Literally, increases the carrying capacity of your body of water! This is awesome for aquaculture setups

Original website
Thank you for sharing!
(1 like)
A hidden gem.

I've had an experience where I had to travel from home for a around a month and I had some of this just finishing up from 2 gallons of milk.  I didn't think the cheese would keep in the fridge the whole time, "first time making it", so I fed some of it to my dog and the rest was just tossed out on the ground in the food forest I was starting.  I went back to loading up my truck and overlooked the fact that I just threw out a heaping stinky cheese curd that was going to attract my dog.  I finished loading my truck, realized what I had done, and yup, he gobbled the rest of it up.  So off we go on a 14 hour drive.  Around 10 hours in, my dog began dry heaving/gagging.  I pull over and we walk around a little bit in a field and I turn around to see him hacking up something stringy....he hacked up a tapeworm.   I've never tried it again to see what would happen if he ate that much of the fermented curd.  But I also wonder if the drive/movement had an additional effect on what had just happened.?

When I was learning about this I read of urban communities in asia where they have polluted canals running down the middle of the streets/walkways.  The community learned of making this and began dumping it into the canals in front of their homes, and the canals cleared up.

I've read that it will keep for a long time in the fridge.  I still have a half gallon that I made early spring, I didn't add any food source for the microbes, I put it in the fridge.  I don't have a microscope to verify that there are still living microbes in there but it looks and smells the same.  The cheese curds are in a separate jar and they look/smell the same as well.

Disease Resistance:

This is a consequence of the increased efficiency of nutrients. More nutrients available at smaller metabolic cost.
Lacto suppresses harmful bacteria

It also suppresses harmful bacteria and fungus like mildew on leaves. I have found that spraying the leaves prevents and suppresses downy and powdery mildew on the leaves 
If I am understanding the recipe correctly, if I started with 1 cup rice wash, I would end up with 200 cups of the final product (1cup rice wash X 10 cups milk X 20 cups water dilution). That is 200 cups, which is 12.5 gallons of the final product that one would use in the sink drain, in the animal water or feed, as an air freshener spray (maybe?), etc. ! Is that right? That would be incredible!
Another question: Does anyone happen to know if essential oils were added to the final dilution, would that kill the beneficial bacteria in the product? My sense is that it would being that essential oils are incredibly powerful, but in case anyone knows for sure either way, I thought I would ask.
Please disregard my question about essential oils and the lacto bacteria. I readily found the answer (and should have done my own research before asking).
Inputs - Section 1 - LAB or Lactic acid bacteria

If you use it on plants what dilution are you using? How much per m2?
What I use is whey that separates from the yogurt I make, usually about 1/4 cup. I put it in a quart or liter spray bottle and fill with water. I spray that on as a fine mist until damp but not dripping. Leaves will vary as to how much they will hold so area covered will vary. Observe what the results are in the next few days. If some leaves shrivel and die but others get green and healthy thtn some leaves may have been infected with a pathogenic agent but the colonization with lacto bacillus has protected other leaves from infestation.
Wink, wink, nudge, nudge, say no more ... richsoil.com/wd-gardening.jsp

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