C. botulinum spores can be killed by heating to extreme temperature (120 degrees Celsius) under pressure using an autoclave or a pressure cooker for at least 30 minutes. The toxin itself can be killed by boiling for 10 minutes.
http://scienceline.ucsb.edu/getkey.php?key=1307#:~:text=C.%20botulinum%20spores%20can%20be%20killed%20by%20heating,can%20be%20killed%20by%20boiling%20for%2010%20minutes.
Because botulinum toxin is destroyed by high temperatures, persons who eat home-canned foods should consider boiling the food for 10 minutes before eating it to ensure safety.
https://emergency.cdc.gov/agent/Botulism/clinicians/control.asp
