Dominik Riva wrote:I'm not sure if I understand you right.
Some principles may help answer your question anyway:
Colonize in stages to minimize contamination. Transfer to the next medium before the mycelium has depleted the nutrients - this will inhibit premature fruiting. Never mix sterilized medium with the next stage/medium/substrate - mix colonized medium with the next stage/medium/substrate.
A typical chain form spore to production looks like this:
Spore print Petri dish Petri dish Petri dish Petri dish - pure strain is archived and samples get labeled and archived in a fridge liquid culture - optional grain grain grain Masters Mix / straw / coffee grounds / wood chips / What ever the fungi can eat
Some additional hints for the chain above:
toss the strains form your archive that didn't do well for you proven strains go from archive fridge to Petri dish from there you fork them back to archive but increase the p-number and to production via grain if production with high p-number strains get weak go back to lower p-numbers of the same strain to refresh the vigor refresh strains in the archive fridge at regular intervals and have a backup off premise
In all of this steps except the spore print the medium needs to be sterilized - Aluminum foil is practically sterile from the heat of production.
The last stage is often sterile but not always as some contamination at the last stage can get easily overwhelmed by the mass of mycelium added - especially if robust fungi like oyster mushrooms are used but can easily reduce yield.
Straw or wood chips or fresh spend coffee grounds get often pasteurized or in the case of coffee ground come pasteurized.
Transfers need to happen as sterile as possible (flow hood or still air box).
The better your sterile practice the more you can dilute the mycelium in the next medium - in the Petri dish stages you always want to get the least amount of material to the the next Petri dish to purify the strain as fast as possible.
Good luck with your business
Dominik Riva wrote:
Bryant RedHawk wrote:
Those containers can be set up to work for growing mycelium and with well thought out holes for the fruits to exit they would make good, reusable containers for growing mushrooms.
You would need to have them in a room set up similar to a clean room to prevent contamination of the growing medium though.
I would advice against the holes and clean room.
It is way easier to keep the contents of the containers sterile then a room.
Install some filter ports for spore free gas exchange - polyfill or cellulose filter disks.
Use the resulting containers to colonize the medium and if pins form dump the block on a rack in a fruiting room - not a clean room but high humidity and ideally HEPA filtered blower that pumps air into the room to produce over pressure.
Be careful not to push the spores from the fruiting room into living quarters and please wear a mask in there as you will get allergic to the spores over time if you don't.
Dominik Riva wrote:Don't think so as the bags need to be air / microbe tight with a filter port for gas exchange.
It would take a lot of work to test for holes and install a filter port.
But the biggest problem: Does the plastic withstand sterilization?