According to the public quality report, my local tap water pH is about 7.8 from over 1000 ft deep well. Hardness is very high at over 250 ppm. The high calcium ions could strengthen the cell walls and slow down leakage. Besides,the indigo plants are grown in full sun so they have thick and tough leaves that remain turgid in water for a long time.
Common 5% white vinegar pH 2.5 is diluted in tap water to reduce pH. I go no more than 1 table spoon to 2 cups for fast small scale extraction. For large scale extraction from mature plants, which I haven't tried this year, I plan on reducing the ratio to 1:100 or lower and soak up to three days. pH in the soaking water would be around 3 to 4.
Beta-glucosidase, the enzyme that breaks down indican into glucose and indoxyl, has optimal activity around pH 5 to 6. The simplest way is to add hydrated lime powder to both increase pH and precipitate indigo pigments (method #1). I prefer adding small amount of washing soda just enough to have indigo forming, then adding calcium for precipitation ( method #2 and #3).
I tested both hydrated lime Ca(OH)2 and calcium chloride for indigo precipitation. The former one results in bulkier aggregates easy for filtering and the latter gives finer and more unstable precipitation. The supernatant from hydrated lime is very alkaline and needs to be neutralized before discarding, while that from soluble calcium is less harsh. Or I can just let the blue water sit for a long time and indigo will settle at the bottom eventually (method #4).
Of the four options, some is faster, some is easier, some requires less material, some gives higher purity. It's personal choice which one to use so just play around and have fun.