Joanne McCartney wrote:John, is it still ok to drink? Will refrigerating it make a difference? I used the remainder to start a new batch, making some more tea, etc. Should I not have used it? Will the yeast affect the scoby? Sorry for all the questions. I'm new to this. It's only my 3rd batch.
You'll only know after you take a sip.
John has given the technical answer, you don't know until you have identified this particular yeast down to species. But unless there has been a history locally of a problem, like a yeast infection that required medication to treat, I would go on the assumption that it is no big problem. I drink orange juice after it has gone fizzy, and I have yet to regret it. Does it give the kombucha an off taste?
This just goes to show that we share the world with the microbes around us. And a lot of the time, we are ingesting those same microbes. Whether a microbe makes a food unsafe to ingest or if it is still "ok", depends on how hungry you are. Blue cheese and natto are two foods taken over by microbes, and they actually improve the health value of the food. On the other hand, meat that is left out at room temperature too long is going to be bad news.
If you refrigerate your fizzy kombucha now, you are going to slow down your yeast culture, perhaps sending them into dormancy -- until you warm them up and add more sugars, then they will be back to making more fizz. And yes, they probably are in your new batch. It's going to be difficult to separate your kombucha critters from the mysterious yeast X, and the best advice would be to start fresh. Maybe someone with better microbiology lab technique than I can make some suggestions.
If you want to avoid this in the future, I would recommend cleaning your kombucha brewing utensils thoroughly with bleach. Homebrewers (of beer) know this well, that bleach is death on yeasts, and you have to rinse especially well or you run the possibility of killing the yeast that you intentionally add to your brew.