Moved a broody hen and her eggs?

The hen didn't sit back on the eggs right away, so I locked her in the nest box. Will she sit again or is it a lost cause?

Depends on the hen.

Give her a little time alone, nothing to lose if you don't have an incubator.

Give her time, she should get her wits about her and go back back to the nest, it's not unusual for a hen to come off her nest to eat, drink, and maybe get a dust bath in. After a hour or so she should be back to normal, if she's been setting for awhile, if she's only a day or two in she may quit the nest. I generally let a hen set for about a week before moving them and then only do my moving at night, very rare to have one quit this way. Usually my problem is trying to keep them from setting, chickens have a strange biological clock.

one of my main hens and her daughter hen will take breaks during sitting and can be seen eating, running, or bathing every few days but they are still 'in the mood' and keep the rooster at bay with an attitude and disposition.

I have a different breed hen who will not get up at all while sitting.

If it was done at night each type would go back to the eggs if moved. If done in daylight type 2 probably wouldn't go back to sitting.