...oy, I lost my last post because of a powerout haha. I'll try my best to summarize.
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It's always a race between molds/yeasts/bacteria and mycellium even after you pressure cook. As a grower, you are trying to make a scenario that favours the mycellium for the longest period of time possible.
As I recall, barley takes longer to cook, but not sure as I've never used it. At the same time, overcooking or oversoaking any grain causes it to crack open or turn mushy which favours mold and bacteria.
To get more detailed information on using barley, try doing a search for "barley tek".
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The RR
video that Joshua linked is a good beginner guide. For future reference, here is what is said about preparing dowel for inoculation:
I soak the dowels for 48 hours to get them hydrated, then load into quart jars 1/2 full and PC for 90 minutes. When cool, inoculate with grain spawn and shake well.
Something to note here is that jar to jar transfers are a tough challenge because you have 2 open jars which are exposed to the mold spores floating around the room. Professionals use a flowhood, which creates a airflow of filtered air, to minimize this risk. If you have a stove, you can preheat it before you do your transfer and then crack the door open a bit. This creates a flow of sterilized air where you can transfer your grains which
should help minimize contamination.
Best of luck