[quote=Seva Tokarev]
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242325/pdf/aem00171-0229.pdf
According to that article, it depends on pH and glucose concentration. If glucose concentration is low or pH level is marginal (if it's lower than 4.6, C. botulinum does not grow at all,) toxin is produced but gas production is not.
According to my calculations, mere tablespoon of 5% vinegar per quart brings pH down to 4.2, and USDA guidelines call for much more vinegar than that.
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This is an example of dangerous or incomplete thinking or communication. The mere tablespoon of 5% vinegar per quart does not address the starting pH. It does not account for what ever liquid or alkaline substances exist inside the chunks that float in the liquid. Further, it is not just glucose, it is the concentration of sugars and or salts that affects the osmolarity of the solution. What's needed is a high enough concentration to create an osmotic pressure that prevents the growth of micro organisms. What's that concentration? I don't know, but it has to include the liquid that is incorporated in all the cells of the plant or animal material in the solution.
Here is how cautious I am: when I made tomatillo salsa for the first time, I had research from U of New Mexico, Las Cruces that said tomatillos were ~3.5 pH. But I was adding onions and chilis, both higher pH, and fresh garlic. The garlic, coming out of the ground is likely to bring the clostridium spores with it (they are ubiquitous in the soil). And it only takes ONE to kill me!
I don't want to die. I DON'T WANT TO DIE! And I don't know these people from Las Cruces, maybe they aren't very smart.... And being an expert carries NO special status with me. Experts are wrong every day, so I adjusted the pH down to 3 using pH paper. And water bathed it an hour.
I think people should be VERY cautious in all high risk activities, which means we need to understand what all the variables are in any given situation.
Sometimes I throw out a quart of chicken broth (pressure canned) just because I thought of the risk of botulism in chicken broth, and am superstitious enough to suspect that if I thought of it, it might be the one bad jar.
As for boiling for one minute, or it was quoted one minute at 160 F, I grew up with people boiling high risk food such as canned green beans for 20 minutes, at sea level. I would not risk my life on 160 F for one minute. When I am having company and I make chicken soup with the home canned broth, I boil that for 20 minutes. And then it crosses my mind that boiling at 5000 feet is not the same temperature as boiling at sea level, where I grew up, and all those people were boiling those green beans...
You just can't be too careful with food preservation. We lost a lot when there was a break in the generations passing the knowledge down, and now that there are young people wanting to learn to preserve their own food, where are they to go to get reliable information?
The links here are a good starting place, but as I said, I am careful even with the advice of the certified experts in the field.
Thekla