r ranson wrote: Parasites on the roosts is another one.
Twice I have been visiting and had the chicken herder complain about birds not roosting/not going to bed, and both times they had a major mite/lice problem.
There are different types of mites, and some of them are deadly (blood mites is what my friend calls one of the deadlier variety.)
So I will back up Ms Ranson's suggestion of a thorough clean up as step 1. I use a veggie oil with a few drops of Tea Tree oil added to it to thoroughly oil the perches. My understanding is that this will smother the mites. Some humans are very sensitive to the odour of Tea Tree oil, but I'm only using 5 drops in about 1 1/2 cups of oil. I agree that too much of anything smelly could hurt delicate chicken lungs, but that's enough mix to do more than two 10 ft perches, and I haven't seen any ill effects beyond the perches being a bit slippery for a couple of days!
Are your birds using their nest boxes? That's the next place to check thoroughly. This is the one place where I prophylactically use Diatomaceous Earth. It too, isn't good for lungs, but I would thoroughly clean the nest boxes, sun solarize them (assuming that big ball in the sky isn't being obliterated by heavy clouds as it is in my neighborhood at the moment) then I sprinkle the DE amongst the nest material. DE like many things, can kill good guys as well as bad guys. Just because it's on the "organic" list, doesn't mean that I think it should be over used, but on my farm, a little in the next boxes seem to be a good help.
Our Gov't website lists the following as symptoms, and sudden death is on the list as the last item:
Infected birds may show 1or many of these signs:
lack of energy, movement or appetite
decreased egg production
swelling around the head, neck and eyes
coughing, gasping for air or sneezing
nervous signs, tremors or lack of coordination
diarrhea
sudden death
Gov't of Canada: protect your flock
Emily, you haven't mentioned an approximate age for the girls that died? We have found that sudden deaths have tended to happen in either quite young birds, or older geriatric birds. Our geriatric deaths often have shown signs of heart failure, but that's a bit subtle. Taking some good pictures if it happens again would be helpful.